Biography:
Li Zhushi, Ph. D of Peking Union Medical College, Tsinghua University. During the doctorate period, mainly focused on the mechanism exploration of medicine-induced apoptosis of leukemia cells. Since graduated, have been working for Sichuan Yuanda Shuyang Pharmaceutical Co., Ltd and Chengdu Institute of Biological Products. Hold the professional title of associate professor. Got years of experience on the development of novel human use vaccines, such as tetravalent Japanese encephalitis/Dengue recombinant vaccine, measles virus-based vaccines, human diploid cell rabies vaccine, EV71 inactivated vaccine and measles/Japanese encephalitis combined attenuated live vaccine. Have also did some fundamental research of Japanese encephalitis virus and Dengue virus and illuminated the key factors in the process of virus attenuation. Have published more than 20 papers in various scientific journals.
Research interests:
Molecular biology, Microbiology, Virology, Vaccine, Biotechnology
Publications:
1. Li ZS, Yang HQ, Yang J, et al. Construction and preliminary investigation of a novel dengue serotype 4 chimeric virus using Japanese encephalitis vaccine strain SA14-14-2 as the backbone. Virus Res. 2014, 191: 10-20.
2. Li ZS, Shi KJ, Guan LY, et al. Activation of p53 by sodium selenite switched human leukemia NB4 cells from autophagy to apoptosis. Oncol Res. 2013, 21(6): 325-331.
3. Li ZS, Shi KJ, Guan LY, et al. ROS leads to MnSOD upregulation through ERK2 translocation and p53 activation in selenite-induced apoptosis of NB4 cells. FEBS lett. 2010, 584(11): 2291-2297.
4. Li ZS, Shi KJ, Guan LY, et al. Downregulation of protein kinase Cα was involved in selenite-induced apoptosis of NB4 cells. Oncol Res. 2010, 19(2): 77-83.
5. Yang HQ, Li ZS, Lin H, et al. A novel dengue virus serotype 1 vaccine candidate based on Japanese encephalitis virus vaccine strain SA14-14-2 as the backbone. Arch Virol. 2016, 161(6): 1517-1526. (co-first author)
6. Li ZS, Lin H, Yang J, et al. Expression of the envelope protein of Dengue virus type 4 and preparation of the polyclonal antibody. Basic & Clinical Medicine. 2012, 32(11): 1337-1341.
7. Li ZS, Shi KJ, Yang Y, et al. Construction of expression vectors for cytochrome c oxidase subunit IV and its overexpression in NB4 Cells. Journal of Qiqihar University of Medicine. 2012, 33(16): 2141-2143.
8. Li Z, Yang Y, Xu C. Mechanism of cytochrome c oxidase subunit IV downregulation in NB4 cells induced by sodium selenite. Journal of Chinese Academy of Medical Sciences. 2009, 31(6):686-691.
9. Li ZS，Yang HQ，Li YH. Causal analysis and resolution of false positive results in reverse transcriptase assay in biological products. Chinese Pharmaceutical Affairs. 2012, 26(12): 1384-1386.
10. Li Z, Xu C. Proteins that regulate cell apoptosis through nucleocytoplasmic transport. Chinese Journal of Biochemistry and Molecular Biology. 2009, 25(10):883-888.
11. Yang J, Yang H, Li Z, et al. Envelope protein mutations L107F and E138K are important for neurovirulence attenuation for Japanese Encephalitis Virus SA14-14-2 strain. Viruses. 2017, 9(1). pii: E20. doi:10.3390/v9010020.
12. Yang J, Yang H, Li Z, et al. The chimeric Japanese encephalitis/Dengue 2 virus protects mice from challenge by both dengue virus and JEV virulent virus. Protein & Cell. 2017, 8(3):225-229.
13. Lin H, Yang H, Li Z, et al. Chimeric Japanese encephalitis /Dengue 3 virus: construction, biological properties and immune response in mice. Molecular Medicine Reports. 2016 (Accepted)
14. Shi K, Jiang Q, Li Z, et al. Sodium selenite alters microtubule assembly and induces apoptosis in vitro and in vivo. J Hematol Oncol. 2013, 6(1): 7.
15. Jiang Q, Wang Y, Li T, Shi K, Li Z, et al. Heat shock protein 90-mediated inactivation of nuclear factor-κB switches autophagy to apoptosis through becn1 transcriptional inhibition in selenite-induced NB4 cells. Mol Biol Cell. 2011, 22(8): 1167-1180.
16. Guan L, Han B, Li Z, et al. Sodium selenite induces apoptosis by ROS-mediated endoplasmic reticulum stress and mitochondrial dysfunction in human acute promyelocytic leukemia NB4 cells. Apoptosis. 2009, 14(2): 218-225.
17. Guan L, Jiang Q, Li Z, et al. The subcellular distribution of MnSOD alters during sodium selenite-induced apoptosis. BMB Rep. 2009, 42(6): 361-366.
18. Guan L, Han B, Li J, Li Z, et al. Exposure of human leukemia NB4 cells to increasing concentrations of selenite switches the signaling from pro-survival to pro-apoptosis. Ann Hematol. 2009, 88(8): 733-742.
19. Yang Y, Huang F, Ren Y, Xing L, Wu Y, Li Z, et al. The anticancer effects of sodium selenite and selenomethionine on human colorectal carcinoma cell lines in nude mice. Oncol Res. 2009, 18(1): 1-8.
20. Guan L, Huang F, Li Z, et al. P53 transcription-independent activity mediates selenite-induced acute promyelocytic leukemia NB4 cell apoptosis. BMB Rep. 2008, 41(10): 745-750.
21. Wang W, Li ZS, Tan S, et al. Prokaryotic expression and immunogenicity of envelope protein domain III of dengue virus type 1. Chinese Journal of Biologicals.（Accepted）
22. Yang J，Yang HQ，Li ZS, et al. The rescue, characterization and stability analysis of infectious clone of live attenuated Japanese encephalitis vaccine strain SA14-14-2 virus. Chinese Journal of Microbiology and Immunology. 2013, 33(2): 117-122.
23. Lin H, Yang HQ, Li ZS, et al. Prokaryotic expression and antibody preparation of E protein of Dengue Virus type 3. Chinese Journal of Microbiology and Immunology. 2013, 33(5): 371-374.
24. Yang J, Li YH, Li ZS, et al. M279K reversion in envelop protein: the effects on virulence of Japanese encephalitis vaccine strain SA14-14-2. Journal of North Sichuan Medical College. 2013, 28(4): 325-330.
25. Wang W, Ding XP, Yang HQ, Li ZS, et al. Prokaryotic expression of E protein of dengue virus type 1 and preparation of polyclonal antibody. Int J Lab Med. 2013, 34(23): 3107-3109.